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Nikon Ni-E Fluorescence Motorized Microscope (New Filters)

Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)
Nikon Ni-E Fluorescence Motorized Microscope (New Filters)

Approved Vendor to many US, Canada, and EU Universities

US & International Shipping

Hundreds of Custom Microscopes  Shipped
Customer References Available 
Training and Installation Options Available

 

  • Stock: In Stock
  • Brand: Nikon
  • Product Code: 57551
  • Condition: Pre-Owned
$21,995.00
CLICKABLE TABS BELOW

For Sale Nikon Ni-E Fluorescence Motorized Microscope

 

  • Fully Functional
  • Serviced & in Stock
  •  Customized to your requirements
  •  Includes new fluorescence filters
  •  Technical sales consultation available
  •  Continental USA 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
  •  Includes remote microscope training session and software overview
  • More info about our Microscopy Capabilities or view the Microscope Buying Guide

This is what you will receive before customizing this microscope:

Standard Nikon Ni-E Fluorescence Motorized Microscope Configuration:

 

Microscopy Techniques:

Brightfield and Fluorescence Microscopy

 

Microscope Base:

  • Nikon Eclipse Ni-E Motorized Upright Base
  • Ni-CTLA Control Box

Microscope Head:

  • C-TF Trinocular Head
  • (2) CFI 10x/22 Eyepieces

Fluorescence Light Path:

  • NI-FLEI EPI-2

Fluorescence Illumination:

  • Exfo X-Cite Metal Halide Lamp w/collimating adapter and light guide

Fluorescence filter cube turret:

  • 6-position Motorized Epi-fluorescence Cube Turret

Fluorescence filters:

  • Three empty filter cubes
  • Three Brand New filter sets of either DAPI, FITC, Texas Red
  • *Other filters available - please inquire*

Brightfield Light Path:

  • Built-in

Brightfield Illumination:

  • LV-LL Nikon LED Lamp w/ NI-CTLA Power Supply

Objective Turret:

  • 6-position NI-ND6-E Motorized Turret

Objectives:

  • 4x/0.13 Plan Fluor 
  • 10x/0.30 Plan Fluor 
  • 20x/0.75 Plan Apo VC (this is a high-quality Nikon APO CFI60 Objective that was originally installed in a custom piece of equipment where the manufacturer did not want to show that they used Nikon objectives)

Condenser:

  • NI-CUD Mechanical Condenser

Stage:

  • Prior Motorized XY Stage
  • Prior Proscan III Control Box w/ Joystick

Camera ports:

  • 100% Eye or 100% Camera

Camera:

  • 8.3MP Color CMOS Camera (we have other CCD and sCMOS cameras we can upgrade to)

Computer & Software:

  • Computer running applicable software

Accessories:

  • Necessary power and communication cables

 

This configuration includes any 3 of these new filter sets.

  • New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
  • New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
  • New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
  • New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)

 

Automated/motorized capabilities of this microscope:

Installed automation/motorized components:

  • Motorized XY Stage
  • Motorized/automated Z-Axis Drive
  • Motorized fluorescence shutter
  • Motorized objective(nosepiece) turret
  • Motorized fluorescence turret

 

Benefits of motorized/Automated Z-Axis drive:

  • Automatic parfocality adjustment
  • The ability to recall previously saved focus levels
  • Automated management of Z positioning/ Fine-coarse control

 

This microscope's imaging capabilities:

Imaging software highlights:

  • Ability to take still images and live videos of your samples
  • Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
  • Variable linear measurements such as line segment, radius values, and others
  • Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
  • Multi-frame (Time lapse/Image acquisition over time)
  • Multi-slice (Z coordinate/Z Stacking)
  • *Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage

 

We can customize this microscope to fit your requirements:

 

Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)

  • Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)

 

Increase Maximum Magnification and Image Quality:

  • Microscope can be configured for magnification between 40x and 200x (with 10x Eyepieces)
  • We can add standard higher magnification Nikon microscope objectives
  • We can add high-end Nikon Apochromat objectives

 

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.

  • Additional filter cubes
  • Additional filter sets to match your fluorochromes.
  • Xenon, Metal Halide, or LED Fluorescence microscope light sources

 

We will also ensure the fluorescence light source matches your selected filter sets.

 

Enhance imaging capabilities:

  • We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

 

We can configure it to work with the sample medium you need:

  • Slide, Petri dish, microplate, etc.

 

Commonly Requested Customer Upgrades

  • High-End CCD, sCMOS Cameras
  • LED or Metal Halide Fluorescence Light Sources
  • Phase Contrast, Polarization, or the DIC configurations
  • Higher Magnification Objectives
  • Higher performance Fluor or APO objectives

 

We are excited to help build and configure the right microscope for you!

 

Nikon Ni-E Upright Microscope Overview

(Final microscope capabilities will depend on the modifications you request)

  • This Nikon Ni-E Fluorescence Motorized Microscope is currently set up for Brightfield with Fluorescence studies.
  • Enhancing components, such as Fluorescence Filter Cubes, have been added.
  • The total current magnification range of this microscope is 40x to 200x (with included objectives)
  • The digital camera is capable of taking detailed digital images.
  • The fluorescence light source included is Exfo X-Cite Metal Halide Lamp.
  • The Nikon Ni-E Fluorescence Motorized Microscope system includes a computer running the software for live feed and still images, and MicroManager software for virtual control.

 

Suited for the following applications as configured:

  • Cell biology: ideal for visualizing live or fixed cells with brightfield or fluorescence markers.
  • Immunofluorescence: detection of specific proteins or structures within cells or tissues using fluorescently labeled antibodies.
  • Molecular biology: visualization of fluorescently tagged nucleic acids or proteins in various experimental setups.
  • Histology/pathology: analysis of stained tissue sections in brightfield and fluorescence modes.
  • Developmental biology: studying organismal or cellular development using fluorescent markers.
  • Neuroscience: imaging fluorescently labeled neurons, synapses, or brain tissue sections.
  • Microbiology: observing microorganisms using fluorescence staining techniques like dapi or gfp tags.
  • Pharmacology/toxicology: testing the effects of drugs on cells or tissues using fluorescent markers to monitor cellular responses.

 

Other potential applications:

  • Live cell imaging
  • Gene expression studies
  • Co-localization studies
  • Fret analysis
  • Frap experiments

 

 

Video Overview:

 

Check out our microscopy capabilities:

https://www.bostonind.com/our-microscopy-capabilities

 

 

How we pack


Why do so many customers choose to order from us?

 

  • We make sure to match the microscope capabilities to your requirements.
  • We have experience servicing and repairing these microscopes.
  • This microscope goes through an extensive set of service steps before shipping.
  • We offer detailed photos and videos of the microscopes you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
  • Many upgrade and repair parts are in stock.
  • Remote installation and microscope operation training are available.
  • On-site installation may be available in the Boston Area (New England area)
  • Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

 

  • Warranty
  • An extended warranty may be available.
  • You get after-sales technical support.
  • Customer references are available.
  • The microscope will be complete and working!
  • Smooth process from the initial quote to order fulfillment.


*SM* Small Crate
260#


 

COMMON CUSTOMER QUESTIONS AND ANSWERS BELOW

 

What steps do you take to verify, service, and confirm the functionality of the Nikon Ni-E Upright Microscope?

We verify various microscope functions individually and together, performing necessary repairs or maintenance to bring the microscope to our quality standards.

 

  • We confirm the microscope's physical condition.
  • We meticulously inspect and clean the optical path within the microscope.
  • We lubricate mechanical components.
  • We assess light source quality using various fluorescence and non-fluorescence samples.
  • We ensure the stage moves smoothly in both fine and coarse adjustments.
  • We ensure the smooth operation of all mechanical parts.
  • We confirm that all electronic/motorized components are working well.
  • We set up communication between the microscope, installed components, and the software.
  • We test the camera by acquiring sample images.
  • We confirm the responsiveness of any included touchscreens and buttons.
  • We ensure necessary accessories and cables are included.

 

Can you help me identify the best microscope and configuration for me?

We are here to help.

  • Contact us with your application; we will help you identify the best microscope configuration.
  • We do not believe in a one-size-fits-all approach; you should get a microscope that will do what you need.

Can I view the Nikon Ni-E Upright Microscope in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the microscope you are interested in.

 

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The microscope is fully warranted for parts and labor.
  • The customer should retain all packing materials during the warranty period.

 

Installation & Training:

 

Option 1 – In Person

If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.

 

Option 2 – Remote/Virtual

Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.

 

Both training options include:

  • Review of the microscope and its capabilities.
  • Microscope and software operation.
  • Review of the recommended maintenance procedures.

 

We generally recommend these training services to anyone who is new to this microscope model.

 

What should I watch out for when buying a Nikon Ni-E Upright Microscope used/pre-owned from another lab?
  • Make sure the microscope is properly packed for shipping and will arrive safely. If the microscope has many components, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

 

Here is what our technical team tests so you can request something similar and ensure your microscope will be fully functional.

  • We confirm the microscope's physical condition.
  • We meticulously inspect and clean the optical path within the microscope.
  • We assess light quality using various fluorescence and non-fluorescence samples.
  • We ensure the stage moves smoothly in both fine and coarse adjustments.
  • We ensure the smooth operation of all mechanical components.
  • We confirm that all electronic/motorized components are working well.
  • We set up communication between the microscope, installed components, and the software.
  • We test the camera by acquiring sample images.
  • We confirm the responsiveness of any included touchscreens and buttons.
  • We ensure necessary accessories and cables are included.

 

We recommend inspecting the microscope in person with your sample or requesting a virtual demo; this will allow you to evaluate the condition and performance of the microscope you are considering purchasing.

 

Do you have a Nikon Ni-E Upright Microscope on hand?
  • Yes, this is a common microscope model we carry, and we stock multiple accessories and many replacement parts to back up our warranty.

 

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this microscope model elsewhere, possibly for less.

 

Here are some of the reasons for our price.

  • Our microscope will be fully serviced.
  • It will be customized and configured to your requirements.
  • We may offer longer standard warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We have to employ technical staff that can do the testing/repairs and after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each microscope we sell.
  • We have to purchase any missing required accessories to make the microscopes complete.
  • We have to procure and stock service parts to fulfill our warranty.
  • We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
  • We guarantee that the microscope will be functional.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

 

How fast will you ship?
  • We can ship within a few days to 2 weeks, depending on your required microscope customization.

 

Will my microscope be insured during shipping?
  • All microscopes shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

How do you pack? Have you previously packed and shipped a Nikon Ni-E Upright Microscope? Do you ship Internationally?

Packing

  • Smaller microscopes ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
  • Most larger microscopes ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile microscopes globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

 

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

 

  • When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your microscope was tested and working the day it left our facility and is covered if there are faults.
Sustainability
  • Well, this one is easy: buying a pre-owned microscope is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

 

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.