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Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope Unit2 Pred Nikon TI-S

Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope Unit2 Pred Nikon TI-S
Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope Unit2 Pred Nikon TI-S
  • Stock: In Stock
  • Brand: Nikon
  • Product Code: 25625
  • Availability:1

Here is a Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope Unit2

Setup for Brightfield, Fluorescence and Phase Contrast studies.
This microscope is optimized for biological studies requiring enhanced contrasting options in all magnifications.
The total magnification of this microscope system is 100 to400x
The XY mechanical stage has a total travel range of 70 x 55mm. This stage will accommodate prepared slides or petri dish.
The 100W mercury lamp will include a new in box 100w bulb (installation required)

Predecessor to:

This Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope is in good working order with all aspects tested and working fully.
Microscope and optics have been professionally cleaned by our technicians.

Please see the exact video and photos below.

Testing we performed:
The Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source fully: PASSED
Tested fluorescence lamp and source: PASSED
Tested port splitter to split between ports as intended: PASSED
Tested mechanical XY stage to limits: PASSED
Tested mechanical nosepiece: PASSED
Tested condenser/prisms: PASSED
Tested objectives for clarity: PASSED
Tested eyepieces for clarity: PASSED
Tested connection to live feed: PASSED
Tested live feed quality: PASSED
Tested all manual shutters: PASSED
Tested mechanical fluorescence slide: PASSED

During testing this Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope was found to be fully functional.
A clear image was produced through the eyepieces as well as the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.

System consists of:
Nikon Diaphot 300 inverted microscope
Binocular Head
(2) CFWN 10x/20 Eyepieces
ELWD 0.30 Phase Contrast condenser
Prisms: Ph1, Ph2
Brightfield Path:
Fluorescence Path:
TE-FM light path w/ manual shutter
Fluorescence Filter Cubes:
3-position Mechanical Slide
B-2A (Blue) EX:450/490 DM:505 EM:520 (F00853)
G-2A (green) EX: 510/560 DM: 575 EM: 590 (F00854)
12V 100W Halogen Lamp w/PSM-1120 Power Supply
LH-M100C-1 Mercury Lamp w/ HB-101013AF Power Supply
6-position Mechanical Nosepiece
10x/0.30 Plan Ph1 (O01563)
20x/0.40 Plan ELWD Ph2 (O01659)
40x/0.56 Plan DL (O02383)
Mechanical XY
Photo/Port Splits:
Eyepieces 100% or Front 80% Eye 20% or Side Port 100% or Side Port 80% Eye 20% or 100% Head
AmScope MU1403 CMOS 14MP Digital Color Camera
Computer and Software:
Dell Latitude i5 running AmScope Software

Communication and power cables.

Configuration and Setup:
Orientation: Inverted
Illumination Type: Brightfield w/ Fluorescence and Phase Contrast
Objectives: 10x/0.30 Plan Ph1, 20x/0.40 Plan ELWD Ph2, 40x/0.56 Plan DL Ph3
Light Sources: 12V 100W Halogen Lamp, 100W Mercury Lamp
Stage: Mechanical XY

The Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope is currently set up for brightfiled with phase contrast and reflective fluorescence studies.
The AmScope 14MP digital camera is capable of taking detailed images and live feed.
The Nikon system includes a Dell Latitude i5 laptop running AmScope software.

Video Description:
We zoom out and get a look at the entire Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope , all components, connections, and serial tags.
Once this inspection is completed all the functions of the scope are demonstrated, all zoom levels phase and fluorescence is shown.
Once we've seen everything, and seen it working, we return to the item tag and the video ends

Datasheet: N/A

Brochure: N/A


These are fully functional and were removed from an automation line of the plant that was shut down.

*SM* Small Crate
OB Packed Weight - 300lb

Please do not hesitate to contact us if you have any questions.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.


All equipment warranty request must have a support case opened within the 60-day Domestic USA and 30-day International warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.

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