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Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope Pred Nikon Ti2

Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope Pred Nikon Ti2
Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope Pred Nikon Ti2
  • Stock: In Stock
  • Brand: Nikon
  • Product Code: 25342
  • Availability:1

Here is a Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope

Setup for Brightfield, Fluorescence and Phase Contrast studies. 
-This microscope system is optimized for biological studies requiring increased contrasting options. 
-Enhancing components, such as PH light annulus have been added 
-Enhancing components, such as Fluorescence Filter Cubes have been added (Other components are available) 
-The total current magnification range of this microscope is 40 to 400x (Other objectives are available) 
-This microscope is compatible with slides and petri dishes. (Options for microplates may be available) 
-The fluorescence light source included is an HBO which uses inexpensive replacement lamps and will arrive with a brand new bulb 
-The included camera software allows for live viewing and image/video capture with variable linear measurements such as line segment, radius values, and others 
-The easy to use interface also allows for customization of color and resolutions settings

Please see the video and photos taken of this exact microscope down below

This Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope is in good working order with all of its aspects tested and fully functional

Optics Inspection:

Microscope and optics have been professionally cleaned by our microscopy technical team.

This inspection is repeated prior to the microscope being packed for shipping to its new destination.

Testing we performed:
The Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp & source fully: PASSED
Tested port splitter to split between ports as intended: PASSED
Tested mechanical XY stage to limits: PASSED
Tested mechanical nosepiece: PASSED
Tested condenser/prisms: PASSED
Tested objectives for clarity: PASSED
Tested eyepieces for clarity: PASSED
Tested connection to live feed: PASSED
Tested live feed quality: PASSED
Tested all manual shutters: PASSED
Tested mechanical fluorescence slide: PASSED
Tested fluorescence lamp and source fully: PASSED

During testing this Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope was found to be fully functional.
A clear image was produced through the eyepieces as well as the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
Halogen light source may be controlled by external power supply only.

System consists of:
Nikon Diaphot 300 inverted microscope
Binocular tube head
(2) CFWN 10x/20 Eyepieces
ELWD 0.30 Phase Contrast condenser
Prisms: Ph1, Ph2, PhL
Brightfield Path:
Fluorescence Filter Cubes:
3-position Mechanical Slide
UV-1A (Blue): EX 365/10, DM 400, EM 420 (#503)
G-2A (Green): EX 530/30, DM 575, EM 590 (#501)
LHS-H100P-1 Halogen lamp w/PSM-1120 Power Supply
LH-M100CB-1 Mercury Lamp w/ HB-101013AF Power Supply
6-position mechanical nosepiece
4x/0.13 Plan DL PhL (#1562)
10x/0.30 Plan Ph1 DL (#2559)
20x/0.4 Plan Ph2 DL (#1564)
40x/0.55 Fluor Ph3 (#O1251)
Manual XY Stage
Metal Stage Insert Disc
Photo/Port Splits:
100% Eyepiece
20/80 Eye/Front Port
100% Side Port
20/80 Eye/Side Port
AmScope MU300 CMOS 3.1MP Digital Color Camera
Computer and Software:
Computer running AmScope Software

Communication and power cables.

Configuration and Setup:
The Nikon Diaphot 300 Inverted Fluorescence Phase Contrast Microscope is currently set up for brightfiled with phase contrast and reflective fluorescence studies.
The AmScope MU300 3.1MP digital camera with the Aptina ultra fine color engine is capable of taking detailed images and live feed.
The Nikon system includes a computer running Windows 10 with AmScope software.

Video Description:

*This video shows the actual microscope in this listing.*

The video provides a walk around view of this microscope and its included components. A functionality demonstration has been performed to show proper operation of the microscope.

Any available additional options functionality has also been demonstrated.

Datasheet: N/A

Brochure: N/A


These are fully functional and were removed from an automation line of the plant that was shut down.

*SM* Small Crate
OB Packed Weight - 300lb

Please do not hesitate to contact us if you have any questions.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.


All equipment warranty request must have a support case opened within the 60-day Domestic USA and 30-day International warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.

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