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Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader w/ Enhanced TRF - AV

Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader w/ Enhanced TRF - AV
Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader w/ Enhanced TRF - AV

Here is a Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader w/ Enhanced TRF

Predecessor to:
Current model

Made in 2020

This Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader is in good working condition.

Please see exact video and photos below.

Testing we performed:
The Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader passed all tests and was found to be fully functional:
Tested power and initialization: PASSED
Tested communication and interfacing with internal software and PC with SoftMax Pro: PASSED
Tested plate carrier movement: PASSED
Tested touchscreen functionality: PASSED
Tested shake function: PASSED
Tested injector functionality: PASSED
Tested Enhanced TRF module: PASSED
Tested absorbance using absorbance test plate: PASSED
Tested fluorescence using flourecence test plate: PASSED
Tested luminescence using luminescent powder in a microplate: PASSED

System consists of:
Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader
SpectraMax Injector System
Enhanced TRF Detection System
Computer running SoftMax Pro software ver7.1.2
Filters Included: FP H VIS (x2), FP V VIS (x2), TRF 616/10 (x2), TRF 490/10, TRF 520/10, TRF 665/10, TRF 360/60, TRF 340/70, L 610/LP, FL 447/60
SpectraDrop Micro-Volume Microplate
NFC Dongles (Qty: 4)
NFC Magnets (Qty: 5)
Standard power and communication cables
USB-C Ethernet Adapter

Configuration and Setup:


Molecular Devices SpectraMax id5 Multi-Mode Microplate Reader


• ABS: DNA/RNA/protein quantification ELISA (HRP, TMB, AP)
• FI: EarlyTo Live Cell (viability), Tryptophan detection, CyQUANT,
Pico/Ribo/Oli Green, NanoOrange, Calcium assays/Cardiomyocyte
beating, Fluorescence injector assays
• Lum: Cell Titer-Glo (cell viability), Dual-LUC reporter assay, MycoAlert, ROS-Glo H2O2 assay
• Fluorescence assays requiring specific filters (NIR)
• FP:IMAP kinase assays
• TR-FRET: HTRF, Cytokine measurement, LanthaScreen, LANCE
• Western blot detection

Read types:

Kinetic, endpoint, spectral scanning, well-area scanning

Detection modes:

• Absorbance
• Fluorescence (top/bottom read)
• Luminescence (top/bottom read)
• Time-Resolved Fluorescence (top/bottom read)
• Fluorescence Polarization (top/bottom read)
• HTRF (top/bottom read)

Wavelength selection:

Hybrid filters and monochromator tunable in 1 nm increments

Wavelength range:

• Abs: 230 - 1000 nm
• FL Ex: 250 - 830 nm
• FL Em: 270 - 850 nm
• Lum: 300 - 850 nm
• TRF Ex: 350 nm
• TRF Em: 450 - 750 nm
• FP: 300 - 750 nm


• Photodiode
• Photo multiplier tube (PMT)

Light Sources:

Xenon Flash Lamp and TRF Laser

Plate format:

6- to 384-well plates (including 96 wells)

Plate read time
96 wells / 384 wells:

• Abs: 28 seconds
• FL: 21 seconds
• Lum: 21 seconds
• TRF: 25 seconds
• FP: 35 seconds


• ABS: < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV; < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
FL: Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well; Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well
• Lum: 2 pM ATP 96 well, 4 pM ATP 384 well
• TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well
• FP: Mono: 2 mP at 10nM 96 well, 2 mP at 10nM 384 well; Filters: 1 mP at 1 nM 96 well, 2 mP at 1 nM 384 well


• Linear
• Orbital
• Double orbital

Injector read modes:

Method independent: Absorbance, Luminescence (all wavelength), Luminescence Monochromator, Fluorescence Intensity top and bottom read modes


Single emission

Detection limit:

50 amol ATP

Dispense volume:

1 uL increments from 1 uL to the maximum allowable volume of the well, based on the selected plate type

Dispense accuracy:

± (4% of volume + 1 uL) / volume x 100%

Dispense precision:

=(2% of volume + 1 uL) / volume [uL] x 100% cv

Dispense speed:

100 uL per second

Dead volume:

50 mL bottle: 1 mL; injector tubing: 250 uL

Temperature Control:

5 DegC above ambient to 66 DegC

Data Analysis Software:

SoftMax Pro software


• Stand alone touchscreen operation
• Near-field communication (NFC) chip functionality enables to pull
up custom protocols with a single tap, saving precious time better spent on research.
• Robotic compatible
• Western Blot
• 2 Injector System
• Enhanced TRF

Power Requirements:

100-240 VAC, 2 A, 50/60 Hz


88.1 lbs. (40 kg)

(H x W x D):

40.1 x 53.2 x 59.8 cm
15.79 x 20.94 x 23.54 in

Video Description:
The video below gives an overview of the Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader, and also demonstrates its functionality.

Datasheet: N/A

Brochure: Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader

Manual: Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader

This will ship via freight. Please contact us with your zip code to obtain shipping quote.

*SM* Small Crate

Please do not hesitate to contact us if you have any questions.

All equipment warranty request must have a support case opened within the 60-day warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.

Every instrument has unique packing and shipping needs. Few instruments can be safely shipped via Pacel, and most require the safety of a well-built crate.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.


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