Eppendorf BioFlo 320 Dual Bioreactor System w/ 10L Vessels, MC 1200 Chiller, DASGIP OD4 & Exhaust Gas Modules

For Sale Eppendorf BioFlo 320 Dual Bioreactor System w/ 10L Vessels, MC 1200 Chiller, DASGIP OD4 & Exhaust Gas Modules

 This item has been recently sold. Please view the other available instruments on your right.  

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• Fully Functional
• Serviced & in Stock
• Current Model
• Technical sales consultation available
• CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
• Includes remote instrument setup and operation training session

Bioreactors are configured to meet individual customer requirements (shown with a 10L glass autoclavable vessel)

We will be able to configure this bioreactor for either microbial production or bioprocessing

Below is the configuration of the two Bioflo 320 controllers you will receive

Compatible vessel types and volumes

Gas flow supply connections

Bioflo 320 Controller digital connections/inputs

Bioflo 320 Controller peristaltic pumps

 Installed liquid connections and their operating temperature ranges  

Controller Weight/Dimensions/Power Requirement

Here is what you will receive:

• 2x Eppendorf Bioflo 320 Bioprocess Control Station (4 built-in Peri Pumps, Direct drive motors) 
• 2x ELO Touchscreen Monitor w/ Arm Mount
• 2x 10L vessels (with a Heating/Cooling base) – Other vessel sizes maybe available
• 2x Stirring drives
• 2x Temperature Sensors 
• 2x PH Sensors
• 2x DO (Dissolved Oxigen) Sensors
• 2x Level/Foam Sensors
• 2x Exhaust gas coolers (w/ quick connect tubing)
• 2x External Watson Marlow 120U Peristaltic Pump (with harness to operate through BioFlo320)
• 1x LAUDA Microcool MC 1200 Circulation chiller 115 V; 60 Hz (with supply/return tubing harness)
• 1x Dasgip OD4 Optical Density module
• 2x Optical Density sensors for 10L vessel
• 2x BlueInOne exhaust O2/CO2 sensors; w/ BlueVis software
• Desktop PC (Monitor, keyboard, mouse, network switch) with the BioCommand software
• All power and communication cables

• The DASGIP OD4 module enables real-time, non-invasive optical density monitoring in up to four vessels. With OD sensors, it tracks biomass growth during microbial processes, helping optimize feeding and harvest timing.

• The LAUDA Microcool MC 1200 Circulation chiller is ideal for temperature control in bioprocessing. When used with the Eppendorf BioFlo 320, it ensures stable thermal conditions for dual bioreactor operations, supporting reliable cell culture and fermentation. With up to 1.2 kW cooling capacity and a -10 to +40 DegC temperature range, it enhances process consistency and efficiency in R&D and scale-up applications.

• The BlueSens exhaust gas cooler with quick-connect tubing cools and dries bioreactor exhaust gases before they reach gas sensors, protecting them from heat and condensation. This setup enables accurate, real-time off-gas analysis (e.g., CO₂, O₂)

• The BioCommand software is designed to enhance your ability to monitor and control your fermentation and cell culture processes through your personal computer. It allows the integration of additional inputs. For example, once configured, the DASGIP OD4 module and Bioflo 320 Bioprocess Control Stations may be monitored by the BioCommand software as a supervisory control and data acquisition (SCADA) software as a complete system.

Bioreactor Vessel Configuration: (2x 10L vessels)

• Bioreactor: autoclavable, stainless-steel dished-bottom, direct drive
• Working volume range: 3.5 - 10.5 L
• Impellers: 2 x Rushton type
• Stainless Baffles
• Sparger Type: ringsparger
• Heated/Cooled base for temperature control

Reactor Customization/Options might be available upon request. Please inquire with our sales team.

• Different volume size vessels
• Choice of agitation impellers (pitched blade, marine, Rushton)
• Redox or CO2 sensors
• Vessel Scale
• Sparge and/or Overlay vessel attachment
• Level / Foam sensors
• Others accesories

Please note that, depending on the requested options, we may sometimes recommend a different model bioreactor that may better fit your application.

Testing performed by our in-house service team:

The BioFlo 320 bioprocess control system passed all tests and was found to be fully functional:

• We confirm communication between all units and the PC, including the BioFlo network, DASGIP USB COM, and BlueInOne COM ports.
• We verify the power supply and complete the system initialization process.
• We assess the functionality of the touchscreen display, confirming responsive and accurate input.
• We test the agitation motor and motor control, confirming smooth and stable operation at multiple RPM ranges.
• We test Pumps 1 through 4 to ensure reliable and accurate delivery rates, including external pumps added to the system.
• We verify the pH sensor readings using a calibration solution, confirming accurate output.
• We verify the dissolved oxygen (DO) sensor readings using a calibration solution, ensuring sensor accuracy.
• We tested OD using test solutions at our selected concentrations.
• We test the heating control system and verify temperature accuracy using a NIST-traceable calibrated probe.
• We test the gas output via the sparge line and verified gas control functionality.
• We test all add-on options and accessories.
• We perform additional testing as requested within reasonable parameters.

Instrument Overview:

• Eppendorf BioFlo 320 Dual Bioreactor System includes two 10L vessels with direct drive motors and Rushton impellers.
• Modular base units with integrated peristaltic pumps and support for left- and right-hand orientation setups.
• Gas mixing via TMFC with 4-gas capability and autoclavable push-connect fittings rated up to 10 psig.
• LAUDA MC 1200 chiller ensures stable temperature control using quick-connect supply and return tubing.
• DASGIP OD4 module enables real-time optical density monitoring with sensors for each 10L vessel.
• Exhaust gas coolers with quick-connect tubing protect analyzers during off-gas CO₂/O₂ monitoring and sterilization.

Instrument Application:

• Microbial fermentation for protein or enzyme production (e.g., E. coli, yeast)
• Biomass growth monitoring and optimization
• Parallel fed-batch process development
• Scale-up studies for biologics manufacturing
• Real-time metabolic profiling via off-gas analysis (CO₂/O₂)
• Temperature-sensitive cell culture experiments with precise thermal control

The Key Features:

• Dual BioFlo 320 controllers with 10L vessels
• LAUDA chiller ensures precise temp control
• DASGIP OD4 tracks real-time biomass growth
• Exhaust gas coolers protect gas sensors
• CO₂/O₂ off-gas analysis for process insight
• Modular design fits fermentation & cell culture

Additional technical info:

• Manual: LAUDA Microcool MC 1200 Circulation Chiller Manual

Video Overview:

About us

How we pack

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• We have experience servicing and repairing these instruments.
  
• This instrument goes through all the described service steps before shipping. see the questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

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