- Stock: In Stock
- Brand: Nikon
- Product Code: 25161
Here is an Electrophysiology Rig w/Patch Clamp w/Nikon Eclipse Ti-S Inverted Fluorescence Microscope w/ dual Sutter MP-285 Micromanipulators and dual Molecular Devices Axon Headstages
Setup for Patch Clamp electrophysiology, Brightfield with Fluorescence, and Phase Contrast studies
- This setup includes (2) Sutter MP-285 micromanipulation systems with Axon Multiclamp headstages.
- The dual headstages allows for simultaneous stimulation and recording (Digitizer and Software not included with this listing)
- A Faraday cage and TMC Antivibration table are included for improved sample recording
- This microscope is optimized for observation of biological studies where enhanced contrasting methods are necessary.
- The XY mechanical stage has a travel range of 70 x 50mm. It will allow for observation of prepared slides and petri dishes.
- The included TMC Anti-Vibration features TMCs Gimbal Piston Air Isolators, allowing quick dissipation of disturbances of the isolated table top and clear imaging without interference from local vibration around the lab.
Please see the video and photos taken of this exact microscope down below
Current model is Ti2
This Nikon Ti-S/L100 Inverted Fluorescence Phase Contrast Microscope is in good working order with all of its aspects tested and fully functional
Microscope and optics have been professionally cleaned by our microscopy technical team.
This inspection is repeated prior to the microscope being packed for shipping to its new destination.
Testing we performed:
The Nikon Ti-S/L100 Inverted Fluorescence Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source fully: PASSED
Tested fluorescence lamp and source fully: PASSED
Tested transmission halogen light path: PASSED
Tested reflective fluorescence light path: PASSED
Tested objectives for clarity: PASSED
Tested port-splitter to split between camera and eyepieces: PASSED
Tested mechanical condenser: PASSED
Tested mechanical Z-axis: PASSED
Tested mechanical fluorescence turret: PASSED
Tested mechanical objective turret: PASSED
Tested mechanical stage: PASSED
Tested Thermo Scientific Photometrics X1 live feed for clarity: PASSED
Tested Sutter MP-285 MicroManipulator: PASSED
During testing this Nikon Ti-S/L100 Inverted Fluorescence Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Eclipse Ti-S/L100 Inverted Microscope
TMC 63-531 Antivibration Table w/ Gimbal Piston Air Vibration Isolation System
TI-TD B Binocular Head
(2) CFI 10x/22 Eyepieces
LWD 0.52 Condenser
Prisms: PhL, Ph1
Fluorescence Turret / Cubes:
TI-FLC 6-position Cube Turret
Qdot EX:425/50 DM:485 BA:655/30
G-2A EX:535/25 DM:575 BA:590
UV-2A EX:355/25 DM:400 BA420
D-LH 12V 100W Halogen Lamp w/ TI-PS100W power supply
EXFO X-Cite 120 Mercury Lamp w/ culminating adapter and light guide
Sutter Stage attachments
Dual Sutter Instruments MP-285 micromanipulation set ups w/ rotating bases
Axon MultiClamp 700A w/ dual headstages (one for stimulation and another for recording)
Objectives / Turret:
6-position Mechanical Nosepiece
4x/0.13 Plan Fluor PhL DL
10x/0.25 Ph1 ADL
20x/0.75 CFI Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
Photo / Port Split:
100% Left or 100% Head
Thermo Scientific Photometrics X1 CCD Camera
Computer and Software:
PC running PVCam software and MultiClamp 700A software
Communication and power cables.
Configuration and Setup:
This Nikon Ti-S/L100 Inverted Fluorescence Phase Contrast Microscope is currently set up for Brightfield with Phase Contrast and Fluorescence studies.
The Nikon system includes a PC to control the PVCam software and MultiClamp 700A software.
With the included objectives 4x,10x,20 and 10x eyepieces the total magnification range of this microscope is between 40x and 200x magnification.
- the Photometrics X1 CCD: High-resolution, thermo-electrically cooled to 0C; 14-bit dynamic range; 2208 x 2208 pixel array; 4.54um pixel size; 75% peak Quantum Efficiency (QE); USB 2.0 (no additional Camera Link or Firewire Cards needed); 1x1, 2x2 or 4x4 binning options.
- the TMC Micro-g Lab Table provides an excellent vibration- free working surface for loads up to 350 lb (160 kg)
Now with modular construction, these tables are recommended for use in such diverse applications as electrophysiology, cell injection, ultramicrotomy, photomicroscopy, scanning tunnel microscopy, and confocal laser scanning microscopy.
- the Type II Faraday Cage offers improved access and simplified assembly.
Designed for shielding in electro-physiology type applications (around 60Hz and harmonics).
The window-shade retracting front panel is easy to operate and causes less disturbance when adjusted.
The front panel may be positioned anywhere between fully opened and closed and stays in position without a fastener.
This cage incorporates a steel frame and bronze-mesh material and mounts to a required full-perimeter enclosure.
Our 40in tall Faraday Cage includes a 2in diameter hole in the base of the side and rear panels.
This feature eases cable interface to the interior of the cage.
- the Sutter MP-285 flagship precision micromanipulator, the motorized MP-285 is affordable yet offers advanced features found in manipulators costing thousands more.
Custom engineered stepping motors, precision cross-roller bearing slides and proprietary worm gear capstan drives form the basis of the watch-like mechanical system.
The controller provides power to the stage motors with a quiet linear power supply to minimize electrical noise radiation in your setup.
Pipette holders and headstages are securely mounted to the MP-285 with one of our several unique and rigid mounting systems.
- the Molecular Device (former Axon Instruments) Multiclamp 700A is a multipurpose computer-controlled amplifier.
It's ideal for many applications, including high-speed current clamp (sharp-electrode or field potentials), patch clamp (whole-cell, macro-patch, or excised patch), voltammetry/amperometry, ion-selective measurements and bilayer recordings.
It supports one or two independent CV-7A headstages enabling simultaneous recording from two cells (channels).
Each headstage contains current-to-voltage conversion as well as voltage-following circuitry, which allow the user to switch between single-electrode voltage clamp and fast current clamp.
This mode switching is very rapid and can be externally triggered, enabling hybrid current and voltage clamp recording during a single experiment.
*This video shows the actual microscope in this listing.*
The video provides a walk around view of this Nikon Ti-S/L100 Inverted Fluorescence Phase Contrast Microscope and its included components.
A functionality demonstration has been performed to show proper operation of the Nikon Ti-S/L100 Inverted Fluorescence Phase Contrast Microscope.
Any available additional options functionality has also been demonstrated.
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*SM* Small Crate
OB Packed Weight - 300lb
Please do not hesitate to contact us if you have any questions.
Here we are showing you our standard packing process
1. All items are different, but they all follow the same principle when packaging.
2. Start with a well-constructed crate
A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.
3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.
4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.
5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.
6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.
7. Final step is to photograph everything inside the crate and then seal it with screws.
All equipment warranty request must have a support case opened within the 60-day Domestic USA and 30-day International warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.