- Stock: In Stock
- Brand: BD
- Product Code: 17908
Here is a BD LSR II Flow Cytometer (3)Lasers/(9)Colors w/ HTS Sampler Pred LSRFortessa
Setup with (3) Lasers: 488nm Blue / 640nm Red / Violet 405nm, and (9) Colors: (4) Blue, (3) Red, (2) Violet
Current model is LSRFortessa X-20 (that is new around 260-380K), so this might be a much better option if you do not have the budget.
This BD LSR II Flow Cytometer is in good working order.
Cytometers must be installed and PM'ed by certified professionals to ensure best performance.
Please see the exact video and photos below.
Testing we performed:
The BD LSR II Flow Cytometer passed all tests and was found to be fully functional:
Tested power on and initialization: PASSED
Tested communication with software: PASSED
Tested fluidics system: PASSED
Collected events with CS&T beads: PASSED
Reviewed gated data collection on all 9 FL channels for clear defined peaks: PASSED
Tested Cytometer Baseline Run with CS&T beads: PASSED
Tested Cytometer Performance Run with CS&T beads: PASSED
Motion test for high throughput sampler (HTS): PASSED
Position test for HTS: PASSED
This BD LSR II Flow Cytometer has been fully tested. All channels tested to have good peaks and good CV's. Lasers have been tested to
have good power output and are stable over 24 hour period. Fluidics system has been throughly cleaned and flow tested. The HTS autosampler
has been tested through full operational range and with setup beads to verify full operation. System passes the CS&T Baseline and Performance
testing in the FACSDiva software. Due to this testing you can purchase with confidence this BD LSR II Flow Cytometer will preform as shown.
System consists of:
BD LSR II Flow Cytometer
HTS High throughput sampler
Sheath fluid container
Waste container with level alarm
Desktop PC running FACSDiva software ver8.0 (w (2) monitors, keyboard and mouse)
Communication and power cables.
Configuration and Setup:
(3) Laser: 488nm Blue / 640nm Red / Violet 405nm
(9) Colors Simultaneously: (4) Blue, (3) Red, (2) Violet
Optics: Forward Scatter Channel 0(488/10)
- 488 nm blue laser: Channel A (780/60BP, 735LP) Cannel B (695/40BP,685LP) Channel C (575/26BP,550LP)
Channel D (530/30BP, 505LP) Channel E (488/10BP) Side Scatter
- 640 nm red laser: Channel A(780/60BP, 735LP), Channel B (710/50BP, 685LP), Channel C (660/20BP)
- 405 nm violet laser: Channel A(525/50BP, 505LP), Channel B (450/50BP)
Fluorochromes Detected: PE-Cy7, PerCP-Cy5.5, PerCP-eF710, PE, Alexa Flour 488, FITC, GFP, APC-Cy7, APC-AF750,
APC-EF780, APC, AF647, AF700, E710, L/D VIOLET, L/D AQUA, AmCyan, and Pacific Blue and too many more to list.
Check with us to verify your dye with the system.
Sample Acquisition Rate: >20,000 events/sec (2 x 107 cells/mL running at 60uL/min)
This video is a walkaround of the BD LSR II Flow Cytometer system, showing the internals and the optics configuration.
The video also shows the workstation and the FACSDiva software version.
The video performs HTS sampling shows the operation of the HTS and data in the FACSDiva software.
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
Please do not hesitate to contact us if you have any questions.
Here we are showing you our standard packing process
1. All items are different, but they all follow the same principle when packaging.
2. Start with a well-constructed crate
A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.
3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.
4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.
5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.
6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.
7. Final step is to photograph everything inside the crate and then seal it with screws.
All equipment warranty request must have a support case opened within the 60-day Domestic USA and 30-day International warranty period.
To open a support case, please send an email to firstname.lastname@example.org or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.