BD FACSCalibur Flow Cytometer w/ Cytek DxP Multi-Color Upgrade 3 Laser/10 Color
BD FACSCalibur Flow Cytometer w/ Cytek DxP Multi-Color Upgrade 3 Laser/10 Color | Priced $28,995.00 | bostonind.com (14074)
Here is a BD FACSCalibur Flow Cytometer w/ Cytek DxP Multi-Color Upgrade 3 Laser/10 Color
New costs over $100,000 + $80,000 for the Cytek system upgrades
PM performed by Cytek on 10/25/2018
Maintenance included alignment of optics, replacement of seals and filters, calibration of fluidics pressures and verification of operation using calibration beads to test all channels.
Predecessor to:
Current model.
It might be great option if you want to save on BD FACSCANTO II and 3 laser version FacsAria BD FACSARIA III
Condition:
This BD FACSCalibur Flow Cytometer is in good working order.
Please see the exact video and photos below.
Testing we performed:
The BD FACSCalibur Flow Cytometer passed all tests and was found to be fully functional:
Tested communications with PC: PASSED
Tested operation of fluidics: PASSED
Tested operation of optics: PASSED
Tested functionality of microsampler: PASSED
The testing performed verifies operation by requiring the instrument to pass calibration. In order to have a passing result, the fluidics system has to work perfectly to have the correct flow rate.
The sampling system has to deliver the calibration beads to the flow cell for calibration to be started. The lasers and optics must be clean, aligned with working detectors and properly operating fluidics to get a passing result.
PM and laser alignment performed by Cytek on 10/25/2018 - complete instrument operation verified.
System consists of:
BD Biosciences FACSCalibur Flow Cytometer
Cytek DxP laser upgrade
Cytek AMS system
Cytek Fluid management unit
Dell Latitude i5 running Windows 7 Pro with CytekAMS 1.0.4 and FlowJo CE 7.5 and FlowJo 10.5 with software License Dongle
Communication and power cables.
Configuration and Setup:
Excitation lasers: Violet 405nm, Blue 488nm and Red 639nm
Sample types: Cells and multiplex bead assays
Optics: FSC, SSC, (FL1 530/30 (555LP), FL2 695/40, FL3 585/42 (555LP) (FL4 740LP (725SP), FL5 666/27 (685SP) ( FL6 695/40 (725SP), (FL7 615/25), FL8 740LP (685SP), FL9 450/50 (480SP) FL10 545/30 (480SP)
Fluorochromes Detected: FITC, GFP, YFP, PE-Cy7, APC-Cy7, PE, PerCP, 7ADD, PerCP-Cy5.5, Alex405, DAPI, PB and Alex430 and more... Be sure to ask if your flurochrome will work and we will verify.
Applications: Cell counting, aiding DNA analysis, Bead Array assays and Immunophenotyping
AMS box with fluid management unit and accompanying wash/waist reservoirs with stand.
Fluid Management system that works from sheath qube containers.
Video Description:
We zoom out to get a full view of the BD FACSCalibur Flow Cytometer. Then we zoom in to the monitor to check the software versions.
After that we do an overall view of the BD FACSCalibur Flow Cytometer and accompanying Cytek equipment to include under the hood and inside the fluid drawer and back out to get another full view.
Documentation:
Datasheet: https://drive.google.com/file/d/0Bz5srQzgsa6uRmtfdTZKSEJDRlE/view?usp=sharing
Brochure: https://drive.google.com/file/d/0Bz5srQzgsa6udjZralZ4UGFZdEU/view?usp=sharing
DxP Brochure: https://drive.google.com/file/d/16MiqWQNVDZhthq1as4Fu_gI1QNsjoac0/view?usp=sharing
Manual: https://drive.google.com/file/d/0Bz5srQzgsa6uSUNiN1ZDNnNuNEE/view?usp=sharing
PM Report: https://drive.google.com/open?id=1jTdDrU53XgorOtSsEol0yPPVtKB758W7
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
Please do not hesitate to contact us if you have any questions.
BD FACSCalibur Flow Cytometer w/ Cytek DxP Multi-Color Upgrade 3 Laser/10 Color