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- Stock: SOLD
- Brand: Agilent
- Product Code: 57147
- Condition: Pre-Owned
For Sale Agilent xCELLigence RTCA eSight Imaging and Impedance System
This item has been recently sold. Please view the other available instruments on your right.
If you do not see what you need, let us know via the “Request Quote” form; we often replenish sold-out items.
- Fully Functional
- Serviced & in Stock
- Made in 2022
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- WARRANTY
Here is what you will receive:
- Agilent xCELLigence RTCA eSight Unit
- Computer running eSight Software v1.2.1 with licenses for Basic, 3D Spheroid Assay, and Immunotherapy
- Required Computer Peripherals (mouse, keyboard, monitor, and power supply cables)
- USB Communication Cable
xCELLigence RTCA eSight System Configuration:
Cradle Configuration:
- Three cradles for E-Plate 96 (impedance measurement only) or E-Plate View 96 (impedance measurement and live-cell imaging)
- Two cradles for other vessels
- Compatible Labware:
- Biosensor E-Plate 96, Biosensor E-Plate View 96
- With 96-LED brightfield module: ANSI/SLAS standard 96-well plate, 6-well plate, 35 mm and 60 mm petri dish, T25 flask
Impedance Measurement:
- Test Signal: 22 mV rms ± 20% with maximum 5 mV DC offset at 10 kHz Ω
- Average Impedance Measurement Speed: 2.88 s (E-Plate 96 and E-Plate View 96)
- Impedance Measurement Accuracy: ± (1.5% + 1 Ω)
- Impedance Measurement Repeatability: 0.8%
- Impedance Dynamic Range: 10 Ω to 5 kΩ
- Electronic Switch Resistance: 2 to 5 Ω
Imaging Specifications:
- Optical Imaging Modes: Brightfield and three fluorescent channels (blue, green, red)
- Fluorescence Excitation/Emission: Red (Ex. 588–596 nm; Em. 612–680 nm), Green (Ex. 473–491 nm; Em. 503–561 nm), Blue (Ex. 381–404 nm; Em. 430–462 nm)
- Fluorescence Light Source: High-power LEDs
- Objective: 10x
- Imaging Resolution: 10x at 1.72 um/pixel
- Field of View 10x: 0.85 x 0.88 mm (E-Plate View 96) and 1.68 x 1.40 mm (ANSI/SLAS standard 96-well plate)
- Camera: 5.0 M pixels Image
- Automated Functions: Autofocus, auto-exposure (brightfield)
- Autofocus Methods: Image-based autofocus
Dimensions (WxDxH): 16.9 x 17.5 x 16.2 in. (43.1 x 44.5 x 41.4 cm)
Weight: 60 lb (27.2 kg)
Customization/Options: N/A
- Objectives: 5x, 20x (Please inquire for options)
- Suitable Thermo Dual Stack Incubator
Testing performed by our in-house service team:
The xCELLigence RTCA eSight System passed all tests and was found to be fully functional:
- We verified the power-on and initialization of the system.
- We validated communication with the software.
- We performed the resistor plate calibration for Stations 1-3.
- We evaluated the E-Plate calibration for Stations 1-3.
- We reviewed the assay scheduling with single-well and multi-well imaging.
- We examined the assay scheduling with microscope slide imaging.
- We checked the imaging using brightfield and RGB filters.
- We confirmed the data export process.
Instrument Overview:
- Integrates real-time cell analysis with impedance-based detection and live-cell imaging in one system.
- Offers brightfield and RGB fluorescence imaging alongside label-free impedance monitoring.
- Supports flexible formats, including multi-well plates and microscope slides, for diverse experimental needs.
- Features multiple analysis stations to enable simultaneous, high-throughput assays.
- Delivers precise tracking of cell behavior, morphology, and proliferation in real time.
- The Agilent xCELLigence RTCA eSight streamlines cellular analysis with advanced imaging and impedance technologies.
Instrument Application:
- Cell characterization/QC
- Immunotherapy/cell killing
- Adhesion
- Receptor signaling
- Live cell imaging
- Impedance measurement
The Key Features:
- Real-time cell analysis with imaging and impedance.
- Integrated brightfield & fluorescence imaging.
- Label-free cell monitoring for kinetics and viability.
- Flexible 96-well plate-based experimental setup.
- High-sensitivity impedance for detailed cell profiling.
- Intuitive software for data acquisition & analysis.
Additional technical info:
- Datasheet: xCELLigence RTCA eSight System Datasheet
- Brochure: xCELLigence RTCA eSight System Brochure
Video Overview:
About us
How we pack
Why do so many customers choose to order from us?
- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
- Warranty
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the xCELLigence RTCA eSight System?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards..
- We verified the power-on and initialization of the system.
- We validated communication with the software.
- We perfomed the resistor plate calibration for Stations 1-3.
- We evaluated the E-Plate calibration for Stations 1-3.
- We reviewed the assay scheduling with single-well and multi-well imaging.
- We examined the assay scheduling with microscope slide imaging.
- We checked the imaging using brightfield and RGB filters.
- We confirmed the data export process.
What supplies do I need?
You will need the consumables starting out:
Can I view the xCELLigence RTCA eSight System in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warrantied for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a xCELLigence RTCA eSight System used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We verified the power-on and initialization of the system.
- We validated communication with the software.
- We perfomed the resistor plate calibration for Stations 1-3.
- We evaluated the E-Plate calibration for Stations 1-3.
- We reviewed the assay scheduling with single-well and multi-well imaging.
- We examined the assay scheduling with microscope slide imaging.
- We checked the imaging using brightfield and RGB filters.
- We confirmed the data export process.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a xCELLigence RTCA eSight System on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped xCELLigence RTCA eSight System Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.