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Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply

Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

$38,995.00
CLICKABLE TABS BELOW

For Sale Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply

Pre-owned instrument

 

  • Fully Functional
  • Current model
  • Serviced & in Stock
  • Good cosmetic condition
  • Warranty
  • An extended warranty option and training may be available.

 

  • Made by Life Technologies, and brand of Thermo Scientific
  • Setup with (4) Lasers and (14) Colors - Max configuration
  • Get it under service agreement with Thermo and you will likely end up paying less than 40% vs buying it new.
  • Buying Service agreement will give you a one year warranty and manufacturers support.

 

Here is what you will receive:

  • Thermo Attune NxT Acoustic Focusing Cytometer AFC2
  • Attune NxT Gen Autosampler
  • Attune NxT Extended Fluid Supply System
  • Desktop PC running Attune NxT Software v5.3 (w/ license dongle, monitor, mouse and keyboard)
  • Communication and power cables
  • FCS Express (1-Year Subscription) RUO version

 

Thermo Attune NxT Acoustic Focusing Cytometer Configuration:

Optics:(4) Lasers, (14) Colors, (16) Detectors

  • Forward Scatter Channel: 488/10 blue laser
  • Side Scatter Channel: 488/10 blue laser
  • 405nm violet laser: 440/50, 512/25, 603/48, 710/50
  • 488nm blue laser: 530/30, 590/40 695/40
  • 561nm yellow laser: 585/16, 620/15, 695/40, 780/60
  • 637nm red laser: 670/14, 720/30, 780/60

 

  • Flow Rate: 12.5 to 1,000 uL/min
  • Sample Analysis Volume: 20ul - 4mL
  • Particle Size Range: 0.5 - 50um
  • Data Acquisition Speed: up to 35,000 events/second with 34 parameters, based on a 10% coincidence rate per poisson statistics
  • Maximum Electronic Speed: 65,000 events/second with all parameters
  • No-wash, no-lyse sample preparation workflow
  • Cycle time: 20-40 min for tube

 

  • Attune NxT Autosampler is compatible with many different plate formats, including 96-well, 384-well, and deep-well plates
  • <42 min for 96-well plate; <180 min for 384-well plate

 

  • Attune NxT External Fluid Supply (EFS) is an accessory for the Acoustic Focusing Cytometer that provides increased focusing fluid and waste capacity, allowing the processing of multiple samples without the need for replenishing the focusing fluid supply or emptying the waste container.
  • The extended capacity of the system with the Attune NxT EFS installed is 10 L for focusing fluid supply and up to 18 L for the waste volume (including 2 L of bleach for biosafety requirements)
  • Electrical input: from Attune NxT Acoustic Focusing Cytometer

 

Customization/Options: N/A

 

Testing performed by our in-house service team:

The Thermo Attune NxT Acoustic Focusing Cytometer passed all tests and was found to be fully functional:

  • We checked the startup and initialization process.
  • We connected The Thermo Attune NxT Acoustic Focusing Cytometer to the software.
  • We tested Attune NxT Sample Pump
  • We tested Attune NxT Acoustics
  • We ran fluidics priming and startup sequences.
  • We conducted several cleaning cycles including long clean and flow cell clean.
  • We tested the functionality of the Auto Sampler.
  • We performed a baseline test with Attune performance beads.
  • We checked its performance with Attune performance beads.
  • We confirmed external fluid supply communication
  • We tested external fluid supply cart pumps and level sensors

 

Instrument Overview:

  • The Thermo Attune NxT Acoustic Focusing Cytometer integrates 4 lasers, 14 colors, and 16 detectors for comprehensive signal analysis.
  • With an included autosampler and external fluid supply system, it facilitates automated sample handling and ensures uninterrupted operation.
  • This advanced cytometer is designed for efficient flow cytometry analysis, making it ideal for research applications requiring high-throughput capabilities.
  • The instrument's precise detection and versatile functionality enable detailed analysis of complex cell populations with ease.
  • Through its advanced features and automation, this cytometer accelerates research processes and enhances data accuracy.

 

Instrument Application:

  • Cell sorting and analysis in immunology research
  • Gene expression profiling in molecular biology
  • Detection of rare cell populations in oncology studies
  • Multiplexed protein expression analysis in drug discovery
  • Characterization of circulating tumor cells in cancer research
  • Immunophenotyping of immune cell subsets in vaccine development

 

The key features:

  • 4 lasers for diverse analysis
  • 14 colors for comprehensive data
  • 16 detectors for high sensitivity
  • Autosampler for efficient workflow
  • External fluid supply for continuous operation
  • Precise and reliable results

 

Additional technical info:

 

Note: Subscription is for the Research Use Only (RUO) version. 

 

 

Video Overview:

 

 

About us

 

How we pack

 

Why should you buy from us?

 

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping.
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Many upgrade and repair parts are in stock.
  • Remote installation and instrument operation training are available. (Is not replacement for Service contract from Thermo)
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

 

  • It will work!
  • Warranty
  • An extended warranty may be available.
  • You get after-sales technical support.
  • Smooth process from the initial quote to order fulfillment.
  • Customer references are available.

 

This will ship via freight. Please contact us with your zip code to obtain shipping quote.

*SM* Small Crate ESQ
200#

 

COMMON QUESTIONS AND ANSWERS BELOW

 

What steps do you take to verify, service, and confirm the functionality of the Thermo Attune NxT Acoustic Focusing Cytometer?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

  • We checked the startup and initialization process.
  • We connected The Thermo Attune NxT Acoustic Focusing Cytometer to the software.
  • We tested Attune NxT Sample Pump
  • We tested Attune NxT Acoustics
  • We ran fluidics priming and startup sequences.
  • We conducted several cleaning cycles including long clean and flow cell clean.
  • We tested the functionality of the Auto Sampler.
  • We performed a baseline test with Attune performance beads.
  • We checked its performance with Attune performance beads.
  • We confirmed external fluid supply communication
  • We tested external fluid supply cart pumps and level sensors

 

What supplies do I need?

You will need the consumables starting out:

 

Can I view the Thermo Attune NxT Acoustic Focusing Cytometer in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or you can request a virtual Zoom demo of the instrument you are interested in.

 

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The instrument comes fully warranted for both parts and labor.
  • The customer should retain all packing materials during the warranty period.

 

What should I watch out for when buying a Thermo Attune NxT Acoustic Focusing Cytometer used/pre-owned from another lab?
  • Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

 

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

  • We checked the startup and initialization process.
  • We connected The Thermo Attune NxT Acoustic Focusing Cytometer to the software.
  • We tested Attune NxT Sample Pump
  • We tested Attune NxT Acoustics
  • We ran fluidics priming and startup sequences.
  • We conducted several cleaning cycles including long clean and flow cell clean.
  • We tested the functionality of the Auto Sampler.
  • We performed a baseline test with Attune performance beads.
  • We checked its performance with Attune performance beads.
  • We confirmed external fluid supply communication
  • We tested external fluid supply cart pumps and level sensors

 

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate its condition and performance before you consider purchasing it.

 

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this Thermo Attune NxT Acoustic Focusing Cytometer elsewhere, possibly for less.

 

Here are some of the reasons for our price.

 

  • We may offer longer warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We need technical staff that can perform testing/repairs and provide after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each instrument we sell.
  • We have to purchase various testing supplies (often expensive) to perform testing.
  • We must purchase any required or missing accessories to complete the instruments.
  • We have to procure and stock service parts to support our warranty.
  • We have extensive experience packing and shipping sensitive instruments.
  • We guarantee that the instrument will be functional.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

 

How fast will you ship?
  • We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

 

Will my instrument be insured during shipping?
  • All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, the responsibility for shipping insurance falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

How do you pack? Have you previously packed and shipped Thermo Attune NxT Acoustic Focusing Cytometer? Do you ship Internationally?

Packing

  • Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
  • Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile instruments globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

 

How much will I save buying it pre-owned, and is it worth it?

A lot, and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

 

  • You will pay less than half the cost of buying a new instrument. Why not spend the leftover funds on another instrument for your lab?
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your instrument was tested and working the day it left our facility and that it is covered if there are faults.
  • Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.

 

Sustainability
  • Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

 

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

 

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies