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Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer (5)Lasers/(19)Colors/(21)Detectors - AV

Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer (5)Lasers/(19)Colors/(21)Detectors - AV
Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer (5)Lasers/(19)Colors/(21)Detectors - AV
  • Stock: In Stock
  • Brand: Beckman Coulter
  • Product Code: 50957
  • Condition: Pre-Owned
$79,995.00
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Here is a Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer (5)Lasers/(19)Colors/(21)Detectors

Made in 2018 last serviced in OCT 2023

New system is around 280,000 new

Predecessor to:
Current model

Condition:
This Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer is in good working condition.

Please see exact photos and video below.

Testing we performed:
The Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer passed all tests and was found to be fully functional:
Tested Power and Communication: PASSED
Startup and Initialization: PASSED
Tested Fludics Prime: PASSED
Tested Fluidics Deep Clean: PASSED
Ran Several Deep Clean Cycles: PASSED
Tested Fluidics Pumps: PASSED
Tested Plate Sample Acquisition: PASSED
Tested Tube Acquisition: PASSED
Completed QC with Passing Results: PASSED

System consists of:
Beckman Coulter CytoFLEX LX Flow Cytometer
Desktop Computer Running CytExpert Software
CytExpert Software License Dongle
Computer with Monitor, Keyboard, and Mouse
CytoFLEX Plate Holder
Sheath Fluid Fluidics Harness
Waste Fluidics Harness
Power and Communication Cables.

Configuration and Setup:
Optics:(5) Lasers, (19) Colors, (21) Detectors
- Forward Scatter Channel
- Side Scatter Channel
- 355nm ultraviolet laser: 405/30, 450/45, 740/35
- 405nm violet laser: 440/45, 525/40, 610/20, 710/50, 780/60
- 488nm blue laser: 525/40, 610/20, 690/50
- 561nm yellow laser: 585/42, 610/20, 675/30, 710/50, 763/43
- 638nm red laser: 660/10, 712/25, 763/43

Sample input formats:
- Single Tube Loader: 5 mL (12 x 75 mm) polystyrene and polypropylene sample tubes 1.5 mL and 2 mL micro-centrifuge sample tubes
Dead Volume: 10 uL
Minimum Sample Volume:100 uL
Carryover: = 1.0%
- Plate format: Flat/U/V bottom standard 96-well plate
Dead Volume: 96-well flat bottom plate - 20 uL; 96-well U bottom plate - 10 uL; 96-well V bottom plate -10 uL
Minimum Sample Volume: 45 uL/well
Maximum Sample Volume:250 uL/well
Carryover: < 0.5%

Signal acquisition speed: 30,000 particles/second with 23 parameters
Sample flow rates: Fixed Flow Rates: 10 uL/min, 30 uL/min, 60 uL/min; Custom Flow Rates: 10 - 240 uL/min in 1 uL increments
Throughput: 10 second acquisition without mixing or backflush: <34 min; 10 second acquisition with 3 second mixing and 3 second backflush: < 47 min.
Sensitivity: <30 MESF for FITC, <10 MESF for PE, <25 MESF for APC
Resolution: <300 nm particles (488 nm side scatter), rCV 3%
Power Requirements: 100-240 VAC, 50/60 Hz, 250 VA
Dimensions (D x W x H): 73.3 x 60.5 x 45.1 cm
Weight: 79 kg

Additional Optics Configuration capability

With the included License dongle the optics are CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer (5)Lasers/(19)Colors/(21)Detectors

The instrument has the capacity for 23 parameters, including 21 for fluorescence detection. The fully activated instrument includes three
fluorescent channels from the 355 nm (UV) laser, five from the 405 nm (Violet) laser, three from the 488 nm (Blue) laser, five from the 561 nm
(Yellow Green) laser, three from the 638 nm (Red) laser, and two from the 808 nm (Infrared) laser. Instruments with as few as 14 fluorescent
channels activated are available with the ability to activate additional parameters as needed by purchasing an activation key.

Two more channels and 808 nm (Infrared) laser can be activated by purchasing license upgrade from Beckman.

Video Description:
The video below gives an overview of the Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer, and also demonstrates its functionality.

Documentation:
Datasheet: Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer

Brochure: Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer

Manual: Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3 Flow Cytometer

This will ship via freight. Please contact us with your zip code to obtain shipping quote.

*ME* Medium Crate ESQ
350#

Please do not hesitate to contact us if you have any questions.

Excitation Laser

Emission Filter (nm)

Channel

Recommended Dyes

Red - 638nm

763/43

RL1

APC-H7, AF750

712/25

RL2

Alexa Fluor 700, APC-R700

660/10

RL3

AF647

Blue - 488nm

525/40

BL1

AF488, CFSE, GFP, Sytox Green, BB515

610/20

BL2

PI

690/50

BL3

PI, PerCP, 7AAD

Yellow - 561nm

763/43

GL1

PE, PE-Cy7, PE-AF750

710/50

GL2

PerCp-Cy5.5, PE, PE-Texas Red
Alexa Fluor 594, mCherry

675/30

GL3

PE, APC, PE-Cy7
AF 647, AF 700

610/20

GL4

PE-Texas Red, PE-Cy5, PI

582/42

GL5

AF555, DsRed, RFP

Violet - 405nm

780/60

VL1

Super Bright 780
Brilliant Violet 786

710/50

VL2

Super Bright 702, Qdot 700

610/20

VL3

Pacific Orange

525/40

VL4

Alexa Fluor® 430/Am Cyan
HorizonBV 510
HorizonV500

440/45

VL5

Cascade Blue®, Pacific BlueTM,
DAPI, Hoechst,
Alexa Fluor® 405, V450

Ultraviolet - 355nm

405/30

UL1

DAPI

450/45

UL2

Hoechst-Blue, DAPI
Pacific Blue
Cascade Blue®

740/35

UL3

GFP, Pacific Blue

FSC

Yes

SSC

Yes

 

 

All equipment warranty request must have a support case opened within the 60-day warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.

Every instrument has unique packing and shipping needs. Few instruments can be safely shipped via Pacel, and most require the safety of a well-built crate.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies