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BD FACSCanto II Flow Cytometer (3)Lasers/(8)Colors/(10)Detectors w/ HTS - AV

BD FACSCanto II Flow Cytometer (3)Lasers/(8)Colors/(10)Detectors w/ HTS - AV
BD FACSCanto II Flow Cytometer (3)Lasers/(8)Colors/(10)Detectors w/ HTS - AV
  • Stock: In Stock
  • Brand: BD
  • Product Code: 48792
  • Condition: Pre-Owned

Here is a BD FACSCanto II Flow Cytometer (3)Lasers/(8)Colors/(10)Detectors with HT Sampler

Predecessor to:
Current model

This BD FACSCanto II Flow Cytometer system is in good working condition.

Please see the exact video and photos below.

Testing we performed:
The BD FACSCanto II Flow Cytometer system passed all tests and was found to be fully functional:
Power on: PASSED
Communication with FACSDiva software: PASSED
Ran fluidics priming and startup sequences: PASSED
Ran several cleaning cycles (long clean, flow cell clean, etc.): PASSED
Tested functionality of HTS: PASSED
Test Run with 8-Peak Rainbow Beads: PASSED
Performed baseline test with CS&T beads: (Beads on Backorder) PASSED
Performed performance check with CS&T beads: (Beads on Backorder) PASSED

System consists of:
BD FACSCanto II Flow Cytometer
BD High-Throughput Sampler (HTS)
BD FACSCanto Fluidics Supply Cart
BD FACSCanto Fluidics Supply Cart Harness
(4) Fluidics Level Sensors (Sheath, Waste, Clean, Shutdown)
Waste Container
Desktop Computer equipped with FACSDiva Software
FACSDiva Software License Dongle
Computer Monitor, Keyboard, and Mouse
Communication Cables and Display Cables

Standard Power Cables

FCS Express (1-Year Subscription) RUO version

Configuration and Setup:
(3) Lasers: 488nm Blue / 633nm Red / Violet 405nm
(8) Colors simultaneously
(10) Detector Channels

- FSC Detector: Photodiode with 488/10 BP; FSC Resolution: 1.0 um
- SSC Detector: PMT with 488/10 BP; SSC Resolution: 0.5 um

Fluorescence Detector Design: Reflective optics with a single transmission filter in front of each PMT
Fluorescence Detectors: 8 PMTs in 4-2-2 standard configuration
- 488nm blue laser: Channel A(780/60, 735LP) Channel B(>670LP,655LP) Channel D(585/42,556LP) Channel E(530/30, 502LP)
- 633nm red laser: Channel A(780/60, 735LP), Channel C (660/20)
- 405nm violet laser: Channel A(510/50, 502LP), Channel B (450/50)
Common Fluorochromes Detected: PE-Cy7, PerCP-Cy5.5, PE, FITC, APC-Cy7, APC, AmCyan, and Pacific Blue (Ask Us if compatible with your planned Fluorochromes)

Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF
Sheath Consumption: 1.10 L/h normal operation; <1 mL/h standby
Carryover: =0.1%
Sample Injection: Direct into flow cell
Max Particle Size: 50 um
Sample Flow Rate, Min: 10 uL/min
Sample Flow Rate, Max: 120 uL/min
Sample Acquisition Rate: 10,000 events/second,
Sample Handling: Manual Tube Loading, High-Throughput Sampler (96-well U-bottom, V-bottom, flat bottom, 364-well flat bottom microplates)
Size (D x W x H) Cytometer: 24 x 36 x 25 in. (61 x 91 x 64 cm)
Weight Cytometer: 320 lb (145 kg)

Video Description:
The video below gives an overview of the BD FACSCanto II Flow Cytometer system and also demonstrates its functionality.

Datasheet: BD FACSCanto II Flow Cytometer

Brochure: BD FACSCanto II Flow Cytometer

Manual: BD FACSCanto II Flow Cytometer

This will ship via freight. Please contact us with your zip code to obtain shipping quote.

Note: Subscription is for the Research Use Only (RUO) version. 

*LO* Long Crate ESQ

Please do not hesitate to contact us if you have any questions.

Excitation Laser

Emission Filter (nm)


Recommended Dyes

Violet - 405nm



V450, VioBlue, AF405, BV421
Cascade Blue, DAPI
DyLight 450, eFluor 450
Marina Blue, Pacific Blue




eFluor 506, CFP, VioGreen
Brilliant Violet 510, Pacific Green
BD Horizon V500

Blue - 488nm



FITC, Alexa Fluor 488








PerCP, PerCP-Cy5.5




PE-CY7, Qdot 800

Red- 633nm



Qdot 655




APC, Alexa Fluor 750
APC-Cy 7, APC-H7






We understand that every instrument has unique packing and shipping requirements; our team understands that no one wants to spend thousands of dollars and receive a damaged instrument.

Our process involves evaluating every instrument to determine the safest shipping method and the best process for packing.

We generally ship parcels either via UPS or freight for all larger goods.

Whenever we ship freight, we use an ISPM-15 wooden crates or pallets made from ½” plywood and 1x4 framing. (Our crates are Internationally Compliant).

On freight shipments, we use 2” 1.7 lb. density foam, which provides excellent cushioning and support for the instrument.

Instruments are safely braced inside the crates. We use solid boards with 1” foam shrink-wrapped around them, which provides cushioning in all directions and holds instruments firmly in place.

Final step is to photograph everything inside the crate and then seal it with screws.



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